This study explored psychological safety climate theory within the modern workplace context of business-to-business sales interactions in the U.S. healthcare industry. Although the measurement of psychological safety has been widely examined in static team environments, a critical gap remains in understanding both how it is created and how it functions in asymmetric, cross-boundary interpersonal interactions characterized by high task interdependence and complex power dynamics. To address this gap, this qualitative study used semistructured interviews with 40 participants (20 customers and 20 salespeople in the healthcare industry) to identify the salesperson behaviors that influence a customer’s perception of psychological safety and the customer behaviors that result. The findings revealed that a psychologically safe climate in the business-to-business sales context is shaped by three complex salesperson behavioral constructs—trust building, respectful cooperation, and strategic patience—each underpinned by the emotional intelligence competencies of empathy, self-regulation, and adaptability. These salesperson behavioral constructs foster customer perceptions of trust and respect that are characteristic of a psychologically safe climate, enabling customer cooperative co-creation behaviors in asymmetrical power relationships where the customer holds formal authority.