Date of this Version
Nucleic Acids Research
Enhancer elements are essential for tissue-specific gene regulation during mammalian development. Although these regulatory elements are often distant from their target genes, they affect gene expression by recruiting transcription factors to specific promoter regions. Because of this long-range action, the annotation of enhancer element–target promoter pairs remains elusive. Here, we developed a novel analysis methodology that takes advantage of Hi-C data to comprehensively identify these interactions throughout the human genome. To do this, we used a geometric distribution-based model to identify DNA–DNA interaction hotspots that contact gene promoters with high confidence. We observed that these promoter-interacting hotspots significantly overlap with known enhancer-associated histone modifications and DNase I hypersensitive sites. Thus, we defined thousands of candidate enhancer elements by incorporating these features, and found that they have a significant propensity to be bound by p300, an enhancer binding transcription factor. Furthermore, we revealed that their target genes are significantly bound by RNA Polymerase II and demonstrate tissue-specific expression. Finally, we uncovered that these elements are generally found within 1 Mb of their targets, and often regulate multiple genes. In total, our study presents a novel high-throughput workflow for confident, genome-wide discovery of enhancer–target promoter pairs, which will significantly improve our understanding of these regulatory interactions.
© The Author(s) 2013. Published by Oxford University Press.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
gene expression, deoxyribonuclease i, DNA, genetic enhancer elements, genes, genome, human genome, histones, mammals, oncogenes, promoter regions (genetics), RNA polymerase ii, transcription factor, candidate disease gene, enhancer of transcription, workflow, datasets
Hwang, Y., Zheng, Q., Gregory, B. D., & Wang, L. (2013). High-Throughput Identification of Long-Range Regulatory Elements and Their Target Promoters in the Human Genome. Nucleic Acids Research, 41 (9), 4835-4846. http://dx.doi.org/10.1093/nar/gkt188
Additional FilesSupplementary_Data_High-throughput identification of long-range regulatory elements.zip (14923 kB)
Date Posted: 14 July 2017
This document has been peer reviewed.