The objective of this research is to explore how the Three Lines of Defense Model (“the Model”) can most effectively be leveraged by banking professionals to foster trustworthiness in the banking industry. The banking industry’s trustworthiness—or untrustworthiness—has the power to positively or adversely impact the lives of individuals, and the power to enhance or diminish global economic stability (Dia, 2011; Egan, 2017; Financial Stability Board, 2010). This research presents an overview of the literature on the Model, and findings gathered from a confidential online survey, reflecting insights from 43 banking professionals with first-hand experience working within the Model. When implemented strategically by dedicated banking professionals, the Three Lines of Defense Model serves as a highly effective framework for fostering trustworthiness in the banking industry.