Department of Microbiology Papers
Date of this Version
It remains unclear whether γδ T cell receptors (TCRs) detect antigens in a manner similar to antibodies or αβ TCRs. Here we show that reactivity between G8 and KN6 γδ TCRs and the MHC class Ib molecule T22 can be transplanted, with retention of wild-type ligand affinity, after en bloc grafting of G8 and KN6 CDR3δ loops in place onto the CDR3α loop of an αβ TCR. We also find that a shared sequence motif within CDR3δ loops of all T22-reactive γδ TCRs binds T22 in energetically distinct fashions, and that T10d, which binds G8 with weak affinity, is converted into a high-affinity ligand by a single point mutation. These results demonstrate an unprecedented autonomy of a single CDR3 loop in antigen recognition.
Adams, Erin J.; Strop, Pavel; Shin, Sunny; Chien, Yueh-Hsiu; and Garcia, K. Christopher, "An Autonomous CDR3δ is Sufficient for γδ T Cell Recognition of the Nonclassical MHC-I T10/T22" (2008). Department of Microbiology Papers. 7.
Date Posted: 09 May 2017
This document has been peer reviewed.
At the time of publication, author Sunny Shin was affiliated with Stanford University School of Medicine. Currently, she is a faculty member at the Department of Microbiology at the University of Pennsylvania.