An Autonomous CDR3δ is Sufficient for γδ T Cell Recognition of the Nonclassical MHC-I T10/T22

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dc.contributor.authorAdams, Erin J
dc.contributor.authorStrop, Pavel
dc.contributor.authorShin, Sunny
dc.contributor.authorChien, Yueh-Hsiu
dc.contributor.authorGarcia, K. Christopher
dc.date2023-05-17T11:49:08.000
dc.date.accessioned2023-05-22T23:55:52Z
dc.date.available2023-05-22T23:55:52Z
dc.date.issued2008-07-01
dc.date.submitted2015-06-22T09:42:07-07:00
dc.description.abstractIt remains unclear whether γδ T cell receptors (TCRs) detect antigens in a manner similar to antibodies or αβ TCRs. Here we show that reactivity between G8 and KN6 γδ TCRs and the MHC class Ib molecule T22 can be transplanted, with retention of wild-type ligand affinity, after en bloc grafting of G8 and KN6 CDR3δ loops in place onto the CDR3α loop of an αβ TCR. We also find that a shared sequence motif within CDR3δ loops of all T22-reactive γδ TCRs binds T22 in energetically distinct fashions, and that T10d, which binds G8 with weak affinity, is converted into a high-affinity ligand by a single point mutation. These results demonstrate an unprecedented autonomy of a single CDR3 loop in antigen recognition.
dc.description.commentsAt the time of publication, author Sunny Shin was affiliated with Stanford University School of Medicine. Currently, she is a faculty member at the Department of Microbiology at the University of Pennsylvania.
dc.identifier.urihttps://repository.upenn.edu/handle/20.500.14332/40578
dc.legacy.articleid1006
dc.legacy.fields10.1038/ni.1620
dc.legacy.fulltexturlhttps://repository.upenn.edu/cgi/viewcontent.cgi?article=1006&context=microbiology&unstamped=1
dc.source.beginpage777
dc.source.endpage784
dc.source.issue7
dc.source.issue7
dc.source.journalDepartment of Microbiology Papers
dc.source.journaltitleNature Immunology
dc.source.peerreviewedtrue
dc.source.statuspublished
dc.source.volume9
dc.subject.otherCell Biology
dc.subject.otherMicrobiology
dc.subject.otherPathogenic Microbiology
dc.subject.otherVirology
dc.titleAn Autonomous CDR3δ is Sufficient for γδ T Cell Recognition of the Nonclassical MHC-I T10/T22
dc.typeArticle
digcom.identifiermicrobiology/7
digcom.identifier.contextkey7243477
digcom.identifier.submissionpathmicrobiology/7
digcom.typearticle
dspace.entity.typePublication
relation.isAuthorOfPublication28557f46-a32a-44f5-a314-3c477772e375
relation.isAuthorOfPublication28557f46-a32a-44f5-a314-3c477772e375
relation.isAuthorOfPublication.latestForDiscovery28557f46-a32a-44f5-a314-3c477772e375
upenn.schoolDepartmentCenterDepartment of Microbiology Papers
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