Institute for Medicine and Engineering Papers
Role of lateral cell–cell border location and extracellular/transmembrane domains in PECAM/CD31 mechanosensation
Date of this Version
Phosphorylation of tyrosine residues on platelet–endothelial cell adhesion molecule-1 (PECAM-1), followed by signal trans- 13 duction events, has been described in endothelial cells following exposure to hyperosmotic and fluid shear stress. However, it is 14 unclear whether PECAM-1 functions as a primary mechanosensor in this process. Utilizing a PECAM-1–null EC-like cell line, we 15 examined the importance of cellular localization and the extracellular and transmembrane domains in PECAM-1 phosphorylation 16 responses to mechanical stress. Tyrosine phosphorylation of PECAM-1 was stimulated in response to mechanical stress in null cells 17 transfected either with full length PECAM-1 or with PECAM-1 mutants that do not localize to the lateral cell–cell adhesion site and 18 that do not support homophilic binding between PECAM-1 molecules. Furthermore, null cells transfected with a construct that 19 contains the intact cytoplasmic domain of PECAM-1 fused to the extracellular and transmembrane domains of the interleukin-2 20 receptor also underwent mechanical stress-induced PECAM-1 tyrosine phosphorylation. These findings suggest that mechano- 21 sensitive PECAM-1 may lie downstream of a primary mechanosensor that activates a tyrosine kinase.
Platelet endothelial adhesion molecule-1, Endothelial mechanotransduction, Hyperosmotic stress, Fluid shear stress
Date Posted: 03 August 2007
This document has been peer reviewed.
Postprint version. Published in Biochemical and Biophysical Research Communications, Volume 320, Issue 4, 2004, pages 1076-81.
Publisher URL: http://dx.doi.org/10.1016/j.bbrc.2004.06.055