Bone regeneration is a rapidly expanding field. Due to this, demand for characterization of bone-like stem cells has been on the rise. This poster presents a project on the effects of sex and age on the stemness of Periosteum-derived Progenitor Cells (PPCs). It was hypothesized that donor age and sex played a role in the stemness of PPCs. To test the hypothesis, it was planned to compare the expression of stem marker genes in patients of different age and sex. Two categories for age were made: young (0-8 years old) and old (14-18 years old) donors. After extracting the cells from donor tissue, RT-PCR was used to measure the expression of stem marker genes. Basic tests were also done using flow cytometry to test its effectiveness in characterization of PPCs. As of now, this investigation showed that there is no statistical difference between samples of different ages, so age does not appear to affect PPC stemness. There were also not enough female samples to run experiments based on sex. However, flow cytometry was proven to be effective in characterizing PPCs. Therefore, the future directions of this project involve characterizing a larger sample of donors with both flow cytometry and RT-PCR to ensure age does not play a role in PPC stemness, while exploring the role of sex in PPC stemness.