The mammalian cochlear nucleus in the auditory brainstem contains specialized neurons called octopus cells that encode temporal features of sound. These cells undergo rapid change during postnatal development, particularly around the time of hearing onset, which for mice, is around 12 days after birth (P12). In parts of the brain, a critical neuromodulatory pathway during development is acetylcholine. This neuromodulation may also be an important factor in the neuronal development of octopus cells. This study assessed how octopus cells respond to acetylcholine receptors during this time of change. Data was obtained from mice of both sexes at developmental ages both before and after hearing onset (P8, P10, P12, P14, P16), and in situ hybridization using complementary RNA probes for Chrna4 and Chrna7 was conducted. It was found that acetylcholine has the potential to impact postnatal development in octopus cells because receptors are present. Levels remain relatively stable between P12 and P16, suggesting that neuromodulation could influence octopus cell computations after hearing onset.