Recent studies in sociophonetic variations and sexuality have been increasingly paying attention to the construction of queer identities in situated conversational contexts. Different from previous experimental investigations of a somehow uniform conceptualization of gay men’s speech, this study locates the performance of two distinct gay personae among Chinese gay couples in online vlogs through sociophonetic variables in Mandarin that have been shown to ideologically link with gender and sexuality. Results from the linear mixed-effects regression models showed that, as compared to gay men with a self-positioning of laogong (husband), gay men who performed a delicate and cute laopo (wife) persona exhibited higher mean pitch, wider pitch range, and more anterior production of the alveolo-palatal /ɕ/. These sociophonetic variations were also found between males and females in the previous literature, implying that gay couples have reappropriated the sex-based variations to create new socio-indexical meanings of ‘husband and wife’ relationships. Moreover, the somehow innovative /s/-fronting found among gay wives in Mandarin Chinese also provided new evidence on /s/ as a semiotic resource for performing gender and sexuality that has been found cross-linguistically.