Electrical breakdown of an axisymmetric, atmospheric pressure air gap between a wire and a plane has been investigated for a gap length of 0.5 mm. 0- and 02- have been identified as the negative ions affecting the discharge development in air, besides electrons and positive ions, and have been included in the electrical breakdown model. Five coupled two-dimensional transient partial differential equations describing the discharge evolution in the air gap have been solved using a finite difference algorithm developed earlier. Temporal development of the charged particle number densities, electrostatic potential, electric field, and current at both the electrodes is presented when the wire is negatively biased at 2500 V. The impact of negative ions on gap breakdown has been assessed by comparing the results of analyses with and without negative ions. It is concluded that the negative ions have negligible effect during the early stages of the discharge development. However, as the discharge evolves, the negative ions cause a net loss of electrons from the discharge. The effect is most pronounced away from the discharge axis, where peaks in the electron density occur as breakdown proceeds. Radial spread of discharge and current growth rate are relatively unaffected by the presence of negative ions, but the magnitude of total current at the electrodes has been found to decrease by a decade when the negative ions are present.