ROLE OF HYPOTHALAMIC CRH NEURONS IN REGULATING THE IMPACT OF STRESS ON MEMORY AND SLEEP

Stress profoundly affects sleep and memory processes. Stress impairs memory consolidation, and similarly, disruptions in sleep compromise memory functions. Yet, the neural circuits underlying stress-induced sleep and memory disturbances are still not fully understood. Here, we show that activation of corticotropin-releasing hormone neurons in the paraventricular nucleus of the hypothalamus (CRHPVN), similar to acute restraint stress, decreases sleep and impairs memory in a spatial object recognition task in male mice. Conversely, inhibiting CRHPVN neurons during stress reduces stress-induced memory deficits while slightly increasing the amount of sleep. We found that both stress and stimulation of CRHPVN neurons activate neurons in the lateral hypothalamus (LH), and that CRHPVN projections to the LH regulate stress-induced memory deficits and sleep disruptions. Our results suggest that CRHPVN neuronal pathways regulate the adverse effects of stress on memory and sleep - an important step toward improving sleep and ameliorating cognitive deficits associated with stress-related disorders.