Buchanan, Sarah A.

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1984 - 19852
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  • Publication
    Mineralization of Short Term Pericardial Cardiac Patch Grafts
    (1984-01-27) Buchanan, James W; Frasca, P.; Soriano, R. Z; Dunn, J. M; Marmon, L.; Buchanan, Sarah A.; Melbin, J.; Chang, S. H; Golub, E. E; Shapiro, I. M
    Glutaraldehyde fixed patch grafts of bovine pericardium were implanted in myocardial windows in young (3-4 months old) sheep. The samples were retrieved after one to three weeks for study with scanning electron microscopy (SEM) and energy dispersive x-ray microanalysis (EDX). A layer of porous material (pseudoneointima, PNI), consisting mostly of a dense mesh of fibers interspersed with blood cells, was noted to form on the blood contacting surface of the graft. Four distinct sets of mineralization were noted in the retrieved grafts: (1) at the blood contacting surface of the PNI; (2) within the PNI at the junction between layers of PNI with differing densities; (#) near the junction of PNI and pericardium (but in the PNI); and (4) within the pericardium. In both the PNI and pericardium the mineral was shown by EDX analysis to contain both calcium and phosphorous indicating the mineral to be a calcium phosphate. Mineralization in the PNI differed from that in the pericardium; in the PNI it was deposited in discrete regions and apparently in association with thrombi while in the pericardium it was distributed diffusely within the collagen matrix, which may influence its formation.
  • Publication
    Morphological Observations of Mineralizing Pericardium Cardiac Grafts
    (1985) Buchanan, James W; Frasca, P.; Soriano, R. Z; Dunn, J. M; Marmon, L.; Buchanan, Sarah A.; Melbin, J.; Golub, E. E; Shapiro, I. M
    Pericardial patch grafters were implanted in the hearts of young sheep for periods ranging from two to 120 days. Explants seven to 21 days old revealed the formation of a "pseudoneointima" (PNI) on the blood contacting surface of the pericardium. The PNI was more heavily mineralized than the pericardium. Mineralization was most intense on the blood contacting surface of the PNI and on the chamber surface of the pericardium. After three weeks of implantation, the PNI was much thinner and was organized into a thin fibrous capsule without any signs of mineralization. In the pericardium, mineral deposits were seen in fibroblasts. Moreover, cell-related mineralization was evident prior to calcification of the surrounding collagen matrix