Departmental Papers (CBE)
Document Type
Conference Paper
Date of this Version
August 2006
Abstract
Delivery of antisense oligonucleotides, AON, presents many of the same challenges as delivery of any nucleic acid: charge, stability, cell uptake, endolysosomal escape, and entry into the nucleus. Here we demonstrate efficient delivery of AON after loading into biodegradable polymer vesicles or 'polymersomes'. We focus on AON delivery to muscle cells in vitro and in vivo because of the emergence of AON in therapeutic strategies directed at muscular dystrophies. To first clarify uptake kinetics without the complications of typical multi-layered myotube cultures, we use micro-patterned C2C12 cells and show efficient uptake of AON-polymersomes. The biodegradable polymersomes break down and foster AON escape with the binding of fluorescent-AON into the nuclear bodies. Intramuscular injections of the polymersome-AON into the hind limbs of mdx-dystrophic mice show more efficient nuclear uptake than AON alone and also lead to dystrophin expression in the mdx mice. In sum, these neutral, degradable carriers of AON show promise in vivo.
Recommended Citation
Kim, Y., Tewari, M., Pajerowski, J. D., Sen, S., Jason, W., Sirsi, S., Lutz, G., & Discher, D. E. (2006). Efficient Nuclear Delivery and Nuclear Body Localization of Antisense Oligo-Nucleotides using Degradable Polymersomes. Retrieved from https://repository.upenn.edu/cbe_papers/112
Date Posted: 09 April 2008
This document has been peer reviewed.
Comments
Copyright 2006 IEEE. Reprinted from Proceedings of the 28th IEEE EMBS Annual International Conference, August 2006, pages 4350-4353.
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