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Here we describe the immunolocalization of a membrane-bound proton pump, the V-type H+-ATPase (VHA), in tissues and isolated cells of scleractinian corals. Immunolocalization of coral proteins requires additional steps not required for various model organisms, such as decalcification of the coral skeleton for immunohistochemistry or removal of cells away from the skeleton for immunocytochemistry. The tissue and cell preparation techniques described here can be adapted for localization of other coral proteins, provided the appropriate validation steps have been taken for the primary antibodies and species of coral used. These techniques are important for improving our understanding of coral cell physiology.
Originally published in Bio-protocol © 2015 Bio-protocol LLC. Reproduced with permission.
Barott, K., & Tresguerres, M. (2015). Immunolocalization of Proteins in Corals: The V-Type H+-ATPase Proton Pump. Bio-protocol, 5 (17), http://dx.doi.org/10.21769/BioProtoc.1573
Date Posted: 04 October 2017
This document has been peer reviewed.