Document Type

Technical Report

Date of this Version

9-5-2015

Publication Source

Bio-protocol

Volume

5

Issue

17

DOI

10.21769/BioProtoc.1573

Abstract

Here we describe the immunolocalization of a membrane-bound proton pump, the V-type H+-ATPase (VHA), in tissues and isolated cells of scleractinian corals. Immunolocalization of coral proteins requires additional steps not required for various model organisms, such as decalcification of the coral skeleton for immunohistochemistry or removal of cells away from the skeleton for immunocytochemistry. The tissue and cell preparation techniques described here can be adapted for localization of other coral proteins, provided the appropriate validation steps have been taken for the primary antibodies and species of coral used. These techniques are important for improving our understanding of coral cell physiology.

Copyright/Permission Statement

Originally published in Bio-protocol © 2015 Bio-protocol LLC. Reproduced with permission.

Comments

At the time of this publication, Dr. Barott was affiliated with the Scripps Institution of Oceanography, but she is now a faculty member of the University of Pennsylvania.

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Date Posted: 04 October 2017

This document has been peer reviewed.