Carbon Nanopipettes Characterize Calcium Release Pathways in Breast Cancer Cells

Loading...
Thumbnail Image
Penn collection
Departmental Papers (MEAM)
Degree type
Discipline
Subject
carbon nano pipes
nanopipette
cell surgery
cell injection
calcium
calcium messenger
Funder
Grant number
License
Copyright date
Distributor
Related resources
Author
Schrlau, Michael G
Brailiou, Eugen
Patel, Sandip
Gogotsi, Yury
Dun, Nae J
Contributor
Abstract

Carbon-based nanoprobes are attractive for minimally-invasive cell interrogation but their application in cell physiology has thus far been limited. We have developed carbon nanopipettes (CNPs) with nanoscopic tips and used them to inject calcium-mobilizing messengers into cells without compromising cell viability. We identify pathways sensitive to cyclic adenosine diphosphate ribose (cADPr) and nicotinic acid adenine dinucleotide phosphate (NAADP) in breast carcinoma cells. Our findings demonstrate the superior utility of CNPs for intracellular delivery of impermeant molecules and, more generally, for cell physiology studies. The CNPs do not appear to cause any lasting damage to cells. Their advantages over the commonly used glass pipettes include smaller size, breakage and clogging resistance, and potential for multifunctionality such as concurrent injection and electrical measurements.

Advisor
Date Range for Data Collection (Start Date)
Date Range for Data Collection (End Date)
Digital Object Identifier
Series name and number
Publication date
2008-07-03
Journal title
Volume number
Issue number
Publisher
Publisher DOI
Journal Issue
Comments
Postprint version. Published in Nanotechnology, Volume 19, Number 32, Article 325102, July 2008, 5 pages. Publisher URL: http://dx.doi.org/http://dx.doi.org/10.1088/0957-4484/19/32/325102
Recommended citation
Collection