Statistics Papers
Document Type
Technical Report
Date of this Version
11-5-2015
Publication Source
Scientific Reports
Volume
5
DOI
10.1038/srep16178
Abstract
Influenza remains a significant cause of disease mortality. The ongoing threat of influenza infection is partly attributable to the emergence of new mutations in the influenza genome. Among the influenza viral gene products, the hemagglutinin (HA) glycoprotein plays a critical role in influenza pathogenesis, is the target for vaccines and accumulates new mutations that may alter the efficacy of immunization. To study the emergence of HA mutations during the course of infection, we employed a deep-targeted sequencing method. We used samples from 17 patients with active H1N1 or H3N2 influenza infections. These patients were not treated with antivirals. In addition, we had samples from five patients who were analyzed longitudinally. Thus, we determined the quantitative changes in the fractional representation of HA mutations during the course of infection. Across individuals in the study, a series of novel HA mutations directly altered the HA coding sequence were identified. Serial viral sampling revealed HA mutations that either were stable, expanded or were reduced in representation during the course of the infection. Overall, we demonstrated the emergence of unique mutations specific to an infected individual and temporal genetic variation during infection.
Copyright/Permission Statement
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Recommended Citation
Cushing, A., Kamali, A., Winters, M., Hopmans, E. S., Bell, J. M., Grimes, S., Xia, L. C., Zhang, N. R., Moss, R. B., Holodniy, M., & Ji, H. P. (2015). Emergence of Hemagglutinin Mutations during the Course of Influenza Infection. Scientific Reports, 5 http://dx.doi.org/10.1038/srep16178
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Business Commons, Influenza Humans Commons, Influenza Virus Vaccines Commons, Statistics and Probability Commons, Virus Diseases Commons
Date Posted: 25 October 2018
This document has been peer reviewed.