Statistics Papers

Document Type

Journal Article

Date of this Version

3-2010

Publication Source

Theoretical Chemistry Accounts

Volume

125

Issue

3

Start Page

593

Last Page

598

DOI

10.1007/s00214-009-0647-4

Abstract

In this study, we investigate whether miRNAs located within “host” protein-coding genes may regulate the expression of their host genes. We find that 43 of 174 miRNAs encoded within RefSeq genes are predicted to target their host genes. Statistical analysis of this phenomenon suggests that gene auto-regulation via miRNAs may be under positive selective pressure. Our analysis also indicates that several of the 43 miRNAs have a much lower expectation of targeting their host genes by chance than others. Among these examples, we identify miR-128b:ARPP-21 (cyclic AMP-regulated phosphoprotein, 21 kD) as a case in which both the miRNA and the target site are also evolutionarily conserved. We provide experimental support for this miRNA:target interaction via reporter silencing assays, and present evidence that this isoform-specific gene auto-regulation has been preserved in vertebrate species in order to prevent detrimental consequences of ARPP-21 over-expression in brain.

Copyright/Permission Statement

The final publication is available at Springer via http://dx.doi.org/10.1007/s00214-009-0647-4.

Keywords

microRNA, ARPP-21, auto-regulation, target

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Date Posted: 27 November 2017

This document has been peer reviewed.