Microfluidic devices have been fabricated with gelatin and paper clips. Optimization of fabrication and characterization process has been carried out systemically by varying gelatin-to-water ratio, bonding time and connector type. We find that the higher gelatin-to-water ratio and the luer lock syringe tip provide a greater success rate, whereas the influence of bonding time is limited. The Reynolds number was calculated to identify whether the fluid shows laminar or turbulent flow.