Department of Microbiology Papers
Document Type
Journal Article
Date of this Version
11-4-1999
Publication Source
AIDS Research and Human Retroviruses
Volume
15
Issue
4
Start Page
329
Last Page
335
DOI
10.1089/088922299311295
Abstract
We surveyed human immunodeficiency virus (HIV) subtype distribution from peripheral blood mononuclear cells (PBMCs) collected in 1995 from 24 HIV-1-infected Kenyan residents (specimens from predominantly male truck drivers and female sex workers near Mombasa and Nairobi). Processed lysates from the PBMC samples were used for env amplification, directly sequenced, and analyzed by phylogenetic analysis. Envelope amplification products were also used for analysis in a polymerase chain reaction (PCR)-based assay, called the combinatorial melting assay (COMA). Results of the two tests were compared for assignment of subtype for this Kenyan cohort. The COMA, a PCR capture technique with colorimetric signal detection, was used with HIV reference subtype strains as well as regional (East Africa) HIV strains for subtype identification. Performance of the COMA was at 100% concordance (24 of 24) as compared with DNA sequencing analysis. Phylogenetic analysis showed 17 isolates to be subtype A, 3 subtype D, and 4 subtype C viruses. This may represent an increase in subtype C presence in Kenya compared with previously documented reports. The COMA can offer advantages for rapid HIV-1 subtype screening of large populations, with the use of previously identified regional strains to enhance the identification of local strains. When more detailed genetic information is desired, DNA sequencing and analysis may be required.
Copyright/Permission Statement
This is a copy of an article published in AIDS Research and Human Retroviruses © 1999 copyright Mary Ann Liebert, Inc.; AIDS Research and Human Retroviruses is available online at: http://www.liebertonline.com.
Recommended Citation
Robbins, Kenneth E.; Kostrikis, Leondios G.; Brown, Teresa M.; Anzala, Omu; Shin, Sunny; Plummer, Francis A.; and Kalish, Marcia L., "Genetic Analysis of Human Immunodefiency Virus Type I Strains in Kenya: A Comparison Using Phylogenetic Analysis and a Combinatorial Melting Assay" (1999). Department of Microbiology Papers. 5.
https://repository.upenn.edu/microbiology/5
Date Posted: 17 June 2015
This document has been peer reviewed.
Comments
At the time of publication, author Sunny Shin was affiliated with the Aaron Diamond AIDS Research Center at Rockefeller University. Currently, she is a faculty member at the Perelman School of Medicine at the University of Pennsylvania.