Alzheimer’s disease (AD) is a progressive neurodegenerative disorder characterized by the accumulation of amyloid-β (Aβ) plaques. The 5xFAD mouse is a transgenic model that develops rapid amyloid pathology, with plaque deposition by 1.5 months and sleep disturbances by 2 months. This study examines Aβ plaque distribution in 2.5-month-old 5xFAD mice to explore potential links between affected brain regions and sleep regulation. Observations revealed that amyloid deposition density varies across different brain regions. These findings may provide insight into the biological mechanisms by which Aβ contributes to cognitive decline and sleep disturbances in AD.