The Maternal Separation with Early Weaning (MSEW model), widely used to study early-life stress and childhood neglect in mice, focuses primarily on offspring outcomes. Its potential to also induce stress in dams, possibly mimicking aspects of postpartum depression (PPD), has not been investigated. We aimed to examine how MSEW affects both dams and their pups, altering maternal behavior and pup development. Understanding these bidirectional effects may provide a more complete picture of mother–offspring dynamics under chronic stress.