Bestrophin Gene Mutations Cause Canine Multifocal Retinopathy: A Novel Animal Model for Best Disease

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dc.contributor.authorGuziewicz, Karina E
dc.contributor.authorZangerl, Barbara
dc.contributor.authorGuziewicz, Karina E
dc.contributor.authorZangerl, Barbara
dc.contributor.authorAguirre, Gustavo D
dc.contributor.authorLindauer, Sarah J
dc.contributor.authorMullins, Robert F
dc.contributor.authorSandmeyer, Lynne S
dc.contributor.authorGrahn, Bruce H
dc.contributor.authorStone, Edwin M
dc.contributor.authorAcland, Gregory M
dc.date2023-05-17T11:19:54.000
dc.date.accessioned2023-05-23T04:44:18Z
dc.date.available2023-05-23T04:44:18Z
dc.date.issued2007-05-01
dc.date.submitted2015-04-08T09:54:19-07:00
dc.description.abstractPURPOSE. Canine multifocal retinopathy (cmr) is an autosomal recessive disorder of multiple dog breeds. The disease shares a number of clinical and pathologic similarities with Best macular dystrophy (BMD), and cmr is proposed as a new large animal model for Best disease. METHODS. cmr was characterized by ophthalmoscopy and histopathology and compared with BMD-affected patients. BEST1 (alias VMD2), the bestrophin gene causally associated with BMD, was evaluated in the dog. Canine ortholog cDNA sequence was cloned and verified using RPE/choroid 5′- and 3′-RACE. Expression of the canine gene transcripts and protein was analyzed by Northern and Western blotting and immunocytochemistry. All exons and the flanking splice junctions were screened by direct sequencing. RESULTS. The clinical phenotype and pathology of cmr closely resemble lesions of BMD. Canine VMD2 spans 13.7 kb of genomic DNA on CFA18 and shows a high level of conservation among eukaryotes. The transcript is predominantly expressed in RPE/choroid and encodes bestrophin, a 580-amino acid protein of 66 kDa. Immunocytochemistry of normal canine retina demonstrated specific localization of protein to the RPE basolateral plasma membranes. Two disease-specific sequence alterations were identified in the canine VMD2 gene: a C73T stop mutation in cmr1 and a G482A missense mutation in cmr2. CONCLUSIONS. The authors propose these two spontaneous mutations in the canine VMD2 gene, which cause cmr, as the first naturally occurring animal model of BMD. Further development of the cmr models will permit elucidation of the complex molecular mechanism of these retinopathies and the development of potential therapies.
dc.identifier.urihttps://repository.upenn.edu/handle/20.500.14332/48861
dc.legacy.articleid1107
dc.legacy.fields10.1167/iovs.06-1374
dc.legacy.fulltexturlhttps://repository.upenn.edu/cgi/viewcontent.cgi?article=1107&context=vet_papers&unstamped=1
dc.source.beginpage1959
dc.source.endpage1967
dc.source.issue103
dc.source.issue5
dc.source.journalDepartmental Papers (Vet)
dc.source.journaltitleInvestigative Ophthalmology & Visual Science
dc.source.peerreviewedtrue
dc.source.statuspublished
dc.source.volume48
dc.subject.otherbiochemistry
dc.subject.othermolecular biology
dc.subject.otherBiochemistry
dc.subject.otherEye Diseases
dc.subject.otherMedical Biochemistry
dc.subject.otherMedical Molecular Biology
dc.subject.otherMolecular Biology
dc.subject.otherOphthalmology
dc.subject.otherOptometry
dc.subject.otherVeterinary Medicine
dc.titleBestrophin Gene Mutations Cause Canine Multifocal Retinopathy: A Novel Animal Model for Best Disease
dc.typeArticle
digcom.contributor.authorisAuthorOfPublication|email:karinag@vet.upenn.edu|institution:University of Pennsylvania|Guziewicz, Karina E
digcom.contributor.authorisAuthorOfPublication|email:bzangerl@vet.upenn.edu|institution:University of Pennsylvania|Zangerl, Barbara
digcom.contributor.authorLindauer, Sarah J
digcom.contributor.authorMullins, Robert F
digcom.contributor.authorSandmeyer, Lynne S
digcom.contributor.authorGrahn, Bruce H
digcom.contributor.authorStone, Edwin M
digcom.contributor.authorAcland, Gregory M
digcom.contributor.authorisAuthorOfPublication|email:gda@vet.upenn.edu|institution:University of Pennsylvania|Aguirre, Gustavo D
digcom.identifiervet_papers/103
digcom.identifier.contextkey6958513
digcom.identifier.submissionpathvet_papers/103
digcom.typearticle
dspace.entity.typePublication
person.identifier.orcid0000-0002-5228-256X
person.identifier.orcid0000-0002-5228-256X
relation.isAuthorOfPublicationf3994ffb-350b-4027-9c2e-e483de443965
relation.isAuthorOfPublication0e009212-6e81-435a-af49-be3ecd21f930
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relation.isAuthorOfPublication.latestForDiscoveryf3994ffb-350b-4027-9c2e-e483de443965
upenn.schoolDepartmentCenterDepartmental Papers (Vet)
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