Allergic contact dermatitis (ACD) is a type IV hypersensitivity mediated by specific antigens that evoke debilitating itch and rash. Traditional patch testing for diagnosing ACD relies on the application of allergen-loaded gel vehicles onto back skin. This process is lengthy, expensive, and inefficient, and the results often contain false positives and negatives. We hypothesized that microneedle patches would offer more consistent and direct allergen delivery to immune cells in the skin and produce more favorable diagnostic outcomes. The microneedle designs were optimized using MATLAB, and the release characteristics of the microneedles were examined ex vivo. The engineered microneedle patches demonstrate rapid and tunable allergen release compared to conventional gel vehicles.