Departmental Papers (Dental)

Document Type

Journal Article

Date of this Version

12-2012

Publication Source

Journal of Periodontal Research

Volume

47

Issue

6

Start Page

711

Last Page

718

DOI

10.1111/j.1600-0765.2012.01485.x

Abstract

Aim

To compare the levels of Selenomonas sputigena and uncultivated/unrecognized Selenomonas species in subgingival biofilms from generalized aggressive periodontitis (GAgP) and periodontaly healthy (PH) subjects.

Material and Methods

GAgP (n=15) and PH (n=15) subjects were recruited and their clinical periodontal parameters were evaluated. Subgingival plaque samples were collected (9 samples/subject) and analyzed for the levels of 10 bacterial taxa, including cultivated and uncultivated/unrecognized microorganisms using the RNA-oligonucleotide quantification technique (ROQT). Differences in the levels of the test taxa between groups were sought using the Mann-Whitney test.

Results

GAgP subjects showed significantly higher mean counts of Porphyromonas gingivalis, Selenomonas sputigena and Selenomonas oral clone CS002 (Human Oral Microbial Database (HOMD) Oral Taxon 131), while Actinomyces gerencseriae and Streptococcus sanguinis were found in higher mean counts in PH subjects (pSelenomonas EW084 (HOMD OT 146) was only detected in the GAgP group. In the GAgP group, levels of P. gingivalis and S. sputigena were higher in sites with probing depth (PD) ≥5mm than in shallow sites (PD ≤3mm) (pP. gingivalis (r=0.77; pS. sputigena (r=0.60; pSelenomonas sp. EW076 (OT 139) (r=042, p<0.05).

Conclusion

S. sputigena, Selenomonas sp. oral CS002 (OT 131) and Selenomonas sp. oral clone EW084 (OT 146) may be associated with the pathogenesis of GAgP, and their role in the onset and progression of this infection should be further investigated.

Copyright/Permission Statement

This is the pre-peer reviewed version of the following article: [Gonçalves, L. F. H., Fermiano, D., Feres, M., Figueiredo, L. C., Teles, F. R. P., Mayer, M. P. A., & Faveri, M. (2012). Levels of selenomonas species in generalized aggressive periodontitis. Journal of Periodontal Research, 47(6), 711-718. doi:10.1111/j.1600-0765.2012.01485.x], which has been published in final form at [http://dx.doi.org/10.1111/j.1600-0765.2012.01485.x]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.

Comments

At the time of publication, author Flavia Teles was affiliated with the Forsyth Institute. Currently, she is a faculty member at the School of Dental Medicine at the University of Pennsylvania.

Keywords

Selenomonas sputigena, molecular biology, 16S rRNA, generalized aggressive periodontitis, not-yet-cultivated species

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Date Posted: 25 February 2022

This document has been peer reviewed.