Departmental Papers (Dental)
Date of this Version
Journal of Periodontal Research
To compare the levels of Selenomonas sputigena and uncultivated/unrecognized Selenomonas species in subgingival biofilms from generalized aggressive periodontitis (GAgP) and periodontaly healthy (PH) subjects.
Material and Methods
GAgP (n=15) and PH (n=15) subjects were recruited and their clinical periodontal parameters were evaluated. Subgingival plaque samples were collected (9 samples/subject) and analyzed for the levels of 10 bacterial taxa, including cultivated and uncultivated/unrecognized microorganisms using the RNA-oligonucleotide quantification technique (ROQT). Differences in the levels of the test taxa between groups were sought using the Mann-Whitney test.
GAgP subjects showed significantly higher mean counts of Porphyromonas gingivalis, Selenomonas sputigena and Selenomonas oral clone CS002 (Human Oral Microbial Database (HOMD) Oral Taxon 131), while Actinomyces gerencseriae and Streptococcus sanguinis were found in higher mean counts in PH subjects (pSelenomonas EW084 (HOMD OT 146) was only detected in the GAgP group. In the GAgP group, levels of P. gingivalis and S. sputigena were higher in sites with probing depth (PD) ≥5mm than in shallow sites (PD ≤3mm) (pP. gingivalis (r=0.77; pS. sputigena (r=0.60; pSelenomonas sp. EW076 (OT 139) (r=042, p<0.05).
S. sputigena, Selenomonas sp. oral CS002 (OT 131) and Selenomonas sp. oral clone EW084 (OT 146) may be associated with the pathogenesis of GAgP, and their role in the onset and progression of this infection should be further investigated.
This is the pre-peer reviewed version of the following article: [Gonçalves, L. F. H., Fermiano, D., Feres, M., Figueiredo, L. C., Teles, F. R. P., Mayer, M. P. A., & Faveri, M. (2012). Levels of selenomonas species in generalized aggressive periodontitis. Journal of Periodontal Research, 47(6), 711-718. doi:10.1111/j.1600-0765.2012.01485.x], which has been published in final form at [http://dx.doi.org/10.1111/j.1600-0765.2012.01485.x]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Selenomonas sputigena, molecular biology, 16S rRNA, generalized aggressive periodontitis, not-yet-cultivated species
Gonçalves, L. F., Fermiano, D., Feres, M., Figueiredo, L. C., Teles, F. R., Mayer, M. P., & Faveri, M. (2012). Levels of Selenomonas Species in Generalized Aggressive Periodontitis. Journal of Periodontal Research, 47 (6), 711-718. http://dx.doi.org/10.1111/j.1600-0765.2012.01485.x
Date Posted: 25 February 2022
This document has been peer reviewed.
At the time of publication, author Flavia Teles was affiliated with the Forsyth Institute. Currently, she is a faculty member at the School of Dental Medicine at the University of Pennsylvania.