Date of this Version
Journal of Clinical Periodontology
To compare the microbiome of healthy (H) and diseased (P) peri-implant sites and determine the core peri-implant microbiome.
Materials and Methods
Submucosal biofilms from 32 H and 35 P sites were analyzed using 16S rRNA sequencing (MiSeq, Illumina), QIIME and HOMINGS. Differences between groups were determined using Principal Coordinate Analysis (PCoA), t-tests and Wilcoxon rank sum test and FDR-adjusted. The peri-implant core microbiome was determined.
PCoA showed partitioning between H and P at all taxonomic levels. Bacteroidetes, Spirochetes and Synergistetes were higher in P, while Actinobacteria prevailed in H (p<0.05). Porphyromonas and Treponema were more abundant in P and while Rothia and Neisseria were higher in H (p<0.05). The core peri-implant microbiome contained Fusobacterium, Parvimonas and Campylobacter sp. T. denticola and P. gingivalis levels were higher in P, as well as F. alocis, F fastidiosum and T. maltophilum (p<0.05).
The peri-implantitis microbiome is commensal-depleted and pathogen-enriched, harboring traditional and new pathogens. The core peri-implant microbiome harbors taxa from genera often associated with periodontal inflammation.
This is the peer reviewed version of the following article: [Sanz‐Martin, I., Doolittle‐Hall, J., Teles, R. P., Patel, M., Belibasakis, G. N., Hämmerle, C. H. F., . . . Teles, F. R. F. (2017). Exploring the microbiome of healthy and diseased peri‐implant sites using illumina sequencing. Journal of Clinical Periodontology, 44(12), 1274-1284. doi:10.1111/jcpe.12788], which has been published in final form at [http://dx.doi.org/10.1111/jcpe.12788]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
microbiome, dental implant, peri-implantitis, DNA, periodontal, sequencing
Sanz-Martin, I., Doolittle-Hall, J., Teles, R. P., Pate, M., Belibasakis, G. N., Hämmerle, C. H., Jung, R. E., & Teles, F. R. (2017). Exploring the Microbiome of Healthy and Diseased Peri-Implant Sites Using Illumina Sequencing. Journal of Clinical Periodontology, 44 (12), 1274-1284. http://dx.doi.org/10.1111/jcpe.12788
Date Posted: 24 February 2022
This document has been peer reviewed.