Departmental Papers (Dental)
Document Type
Journal Article
Date of this Version
12-2017
Publication Source
Journal of Clinical Periodontology
Volume
44
Issue
12
Start Page
1274
Last Page
1284
DOI
10.1111/jcpe.12788
Abstract
Aim
To compare the microbiome of healthy (H) and diseased (P) peri-implant sites and determine the core peri-implant microbiome.
Materials and Methods
Submucosal biofilms from 32 H and 35 P sites were analyzed using 16S rRNA sequencing (MiSeq, Illumina), QIIME and HOMINGS. Differences between groups were determined using Principal Coordinate Analysis (PCoA), t-tests and Wilcoxon rank sum test and FDR-adjusted. The peri-implant core microbiome was determined.
Results
PCoA showed partitioning between H and P at all taxonomic levels. Bacteroidetes, Spirochetes and Synergistetes were higher in P, while Actinobacteria prevailed in H (p<0.05). Porphyromonas and Treponema were more abundant in P and while Rothia and Neisseria were higher in H (p<0.05). The core peri-implant microbiome contained Fusobacterium, Parvimonas and Campylobacter sp. T. denticola and P. gingivalis levels were higher in P, as well as F. alocis, F fastidiosum and T. maltophilum (p<0.05).
Conclusion
The peri-implantitis microbiome is commensal-depleted and pathogen-enriched, harboring traditional and new pathogens. The core peri-implant microbiome harbors taxa from genera often associated with periodontal inflammation.
Copyright/Permission Statement
This is the peer reviewed version of the following article: [Sanz‐Martin, I., Doolittle‐Hall, J., Teles, R. P., Patel, M., Belibasakis, G. N., Hämmerle, C. H. F., . . . Teles, F. R. F. (2017). Exploring the microbiome of healthy and diseased peri‐implant sites using illumina sequencing. Journal of Clinical Periodontology, 44(12), 1274-1284. doi:10.1111/jcpe.12788], which has been published in final form at [http://dx.doi.org/10.1111/jcpe.12788]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Keywords
microbiome, dental implant, peri-implantitis, DNA, periodontal, sequencing
Recommended Citation
Sanz-Martin, I., Doolittle-Hall, J., Teles, R. P., Pate, M., Belibasakis, G. N., Hämmerle, C. H., Jung, R. E., & Teles, F. R. (2017). Exploring the Microbiome of Healthy and Diseased Peri-Implant Sites Using Illumina Sequencing. Journal of Clinical Periodontology, 44 (12), 1274-1284. http://dx.doi.org/10.1111/jcpe.12788
Date Posted: 24 February 2022
This document has been peer reviewed.
Comments
At the time of publication, author Flavia Teles was affiliated with the University of North Carolina at Chapel Hill School of Dentistry. Currently, she is a faculty member at the School of Dental Medicine at the University of Pennsylvania.