Exploring the Microbiome of Healthy and Diseased Peri-Implant Sites Using Illumina Sequencing

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Departmental Papers (Dental)
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microbiome
dental implant
peri-implantitis
DNA
periodontal
sequencing
Dentistry
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Sanz-Martin, I.
Doolittle-Hall, J.
Teles, R. P.
Pate, M.
Belibasakis, G. N.
Hämmerle, C. H.
Jung, R. E.
Teles, F. R.F.
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Abstract

Aim To compare the microbiome of healthy (H) and diseased (P) peri-implant sites and determine the core peri-implant microbiome. Materials and Methods Submucosal biofilms from 32 H and 35 P sites were analyzed using 16S rRNA sequencing (MiSeq, Illumina), QIIME and HOMINGS. Differences between groups were determined using Principal Coordinate Analysis (PCoA), t-tests and Wilcoxon rank sum test and FDR-adjusted. The peri-implant core microbiome was determined. Results PCoA showed partitioning between H and P at all taxonomic levels. Bacteroidetes, Spirochetes and Synergistetes were higher in P, while Actinobacteria prevailed in H (p<0.05). Porphyromonas and Treponema were more abundant in P and while Rothia and Neisseria were higher in H (p<0.05). The core peri-implant microbiome contained Fusobacterium, Parvimonas and Campylobacter sp. T. denticola and P. gingivalis levels were higher in P, as well as F. alocis, F fastidiosum and T. maltophilum (p<0.05). Conclusion The peri-implantitis microbiome is commensal-depleted and pathogen-enriched, harboring traditional and new pathogens. The core peri-implant microbiome harbors taxa from genera often associated with periodontal inflammation.

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2017-12-01
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Journal of Clinical Periodontology
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At the time of publication, author Flavia Teles was affiliated with the University of North Carolina at Chapel Hill School of Dentistry. Currently, she is a faculty member at the School of Dental Medicine at the University of Pennsylvania.
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