Date of this Version
Biochemical and Biophysical Research Communications
The masticatory apparatus absorbs high occlusal forces, but uncontrolled parafunctional or orthodontic forces damage periodontal ligament (PDL), cause pulpal calcification, pulp necrosis and tooth loss. Morphology and functional differentiation of connective tissue cells can be controlled by mechanical stimuli but effects of uncontrolled forces on intra-pulpal homeostasis and ability of dental pulp stem cells (DPSCs) to withstand direct external forces are unclear. Using dynamic hydrostatic pressure (HSP), we tested the hypothesis that direct HSP disrupts DPSC survival and odontogenic differentiation. DPSCs from four teenage patients were subjected to HSP followed by assessment of cell adhesion, survival and recovery capacity based on odontogenic differentiation, mineralization and responsiveness to bone morphogenetic protein-2 (BMP-2). HSP down-regulated DPSC adhesion and survival but promoted differentiation by increasing mineralization, in vivo hard tissue regeneration and BMP-2 responsiveness despite reduced cell numbers. HSP-treated DPSCs displayed enhanced odontogenic differentiation, an indication of favorable recovery from HSP-induced cellular stress.
© <2009>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Dental pulp stem cells; hydrostatic pressure; pulp calcification; tooth; cellular stress
Yu, V., Damek-Poprawa, M., Nicoll, S., & Akintoye, S. O. (2009). Dynamic Hydrostatic Pressure Promotes Differentiation of Human Dental Pulp Stem Cells. Biochemical and Biophysical Research Communications, 386 (4), 645-649. http://dx.doi.org/10.1016/j.bbrc.2009.06.106
Date Posted: 10 August 2018
This document has been peer reviewed.