Departmental Papers (Dental)

Document Type

Journal Article

Date of this Version

2-2017

Publication Source

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

Volume

123

Issue

2

Start Page

163

Last Page

172

DOI

10.1016/j.oooo.2016.09.011

Abstract

Objectives

Mesenchymal stem cells (MSCs) are promising therapies in dentistry due to their multipotent properties. Selecting donor MSCs is crucial because beagle dogs (canines) commonly used in pre-clinical studies have shown variable outcomes and it is unclear whether canine MSCs (cMSCs) are skeletal site-specific. This study tested whether jaw and long bone cMSCs have disparate in vitro and in vivo multilineage differentiation capabilities.

Study Design

Primary cMSCs were isolated from mandible (M-cMSCs) and femur (F-cMSCs) of four healthy Beagle dogs. Femur served as non-oral control. Clonogenic and proliferative abilities were assessed. In vitroosteogenic, chondrogenic, adipogenic and neural multilineage differentiation were correlated with in vivobone regeneration and potential for clinical applications.

Results

M-cMSCs displayed two-fold increase in clonogenic and proliferative capacities relative to F-cMSCs (p =0.006). M-cMSCs in vitro osteogenesis based on alkaline phosphatase (p =0.04), bone sialoprotein (p =0.05), and osteocalcin (p =0.03), as well as adipogenesis (p =0.007), and chondrogenesis (p =0.009) were relatively higher and correlated with enhanced M-cMSC bone regenerative capacity. Neural expression markers, nestin and βIII-tubulin were not significantly different.

Conclusions

The enhanced differentiation and bone regenerative capacity of mandible MSCs may make them favorable donor graft materials for site-specific jaw bone regeneration.

Copyright/Permission Statement

This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/

Keywords

Jaw bone, mesenchymal stem cells, tissue regeneration, dogs

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Date Posted: 10 August 2018

This document has been peer reviewed.