Departmental Papers (Dental)
Precision Targeting of Bacterial Pathogen Via Bi-Functional Nanozyme Activated by Biofilm Microenvironment
Date of this Version
Human dental caries is an intractable biofilm-associated disease caused by microbial interactions and dietary sugars on the host's teeth. Commensal bacteria help control opportunistic pathogens via bioactive products such as hydrogen peroxide (H2O2). However, high-sugar consumption disrupts homeostasis and promotes pathogen accumulation in acidic biofilms that cause tooth-decay. Here, we exploit the pathological (sugar-rich/acidic) conditions using a nanohybrid system to increase intrinsic H2O2 production and trigger pH-dependent reactive oxygen species (ROS) generation for efficient biofilm virulence targeting. The nanohybrid contains glucose-oxidase that catalyzes glucose present in biofilms to increase intrinsic H2O2, which is converted by iron oxide nanoparticles with peroxidase-like activity into ROS in acidic pH. Notably, it selectively kills Streptococcus mutans (pathogen) without affecting Streptococcus oralis (commensal) via preferential pathogen-binding and in situ ROS generation. Furthermore, nanohybrid treatments potently reduced dental caries in a rodent model. Compared to chlorhexidine (positive-control), which disrupted oral microbiota diversity, the nanohybrid had significant higher efficacy without affecting soft-tissues and the oral-gastrointestinal microbiomes, while modulating dental health-associated microbial activity in vivo. The data reveal therapeutic precision of a bi-functional hybrid nanozyme against a biofilm-related disease in a controlled-manner activated by pathological conditions. © 2020 The Authors
Biofilm, Catalytic nanoparticles, Dental caries, Glucose oxidase, Hybrid nanozyme, Polymicrobial
Huang, Y., Yuan, L., Shah, S., Kim, D., Simon-Soro, A., Ito, T., Hajfathalian, M., Yong, L., Hsu, J. C., Neives, L. M., Alawi, F., & Naha, P. C. (2021). Precision Targeting of Bacterial Pathogen Via Bi-Functional Nanozyme Activated by Biofilm Microenvironment. Biomaterials, 268 http://dx.doi.org/10.1016/j.biomaterials.2020.120581
Date Posted: 10 February 2023
This document has been peer reviewed.