Departmental Papers (Dental)
Document Type
Journal Article
Date of this Version
1-6-2018
Publication Source
International Journal of Molecular Sciences
Volume
19
Issue
1
Start Page
Article number 168
DOI
10.3390/ijms19010168
Abstract
Neural EGFL like 1 (Nell-1) is essential for chondrogenic differentiation, maturation, and regeneration. Our previous studies have demonstrated that Nell-1‘s pro-chondrogenic activities are predominantly reliant upon runt-related transcription factor 3 (Runx3)-mediated Indian hedgehog (Ihh) signaling. Here, we identify the nuclear factor of activated T-cells 1 (Nfatc1) as the key transcriptional factor mediating the Nell-1 → Runx3 signal transduction in chondrocytes. Using chromatin immunoprecipitation assay, we were able to determine that Nfatc1 binds to the -833--810 region of the Runx3-promoter in response to Nell-1 treatment. By revealing the Nell-1 → Nfatc1 → Runx3→Ihh cascade, we demonstrate the involvement of Nfatc1, a nuclear factor of activated T-cells, in chondrogenesis, while providing innovative insights into developing a novel therapeutic strategy for cartilage regeneration and other chondrogenesis-related conditions. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
Keywords
Chondrogenesis, Neural EGFL like 1 (Nell-1), Nuclear factor of activated T-cells 1 (Nfatc1), Runt-related transcription factor 3 (Runx3), Adipose Tissue, Animals, Calcium-Binding Proteins, Cell Differentiation, Cells, Cultured, Chondrocytes, Chondrogenesis, Core Binding Factor Alpha 1 Subunit, Core Binding Factor Alpha 2 Subunit, Core Binding Factor Alpha 3 Subunit, Glycoproteins, Mice, Mice, Knockout, NFATC Transcription Factors, Promoter Regions, Genetic, Protein Binding, RNA Interference, RNA, Small Interfering, Signal Transduction, Up-Regulation, neural egfl like 1, nucleic acid binding protein, protein Patched 1, scleroprotein, short hairpin RNA, transcription factor FOXP1, transcription factor FOXP2, transcription factor NFAT, transcription factor NFAT5, transcription factor RUNX1, transcription factor RUNX3, transcription factor Sox1, transcription factor Sox10, transcription factor Sox2, transcription factor Sox8, transcription factor Sox9, unclassified drug, calcium binding protein, glycoprotein, Nell1 protein, mouse, protein binding, Runx1 protein, mouse, Runx2 protein, mouse, Runx3 protein, mouse, small interfering RNA, transcription factor NFAT, transcription factor RUNX1, transcription factor RUNX2, transcription factor RUNX3, animal cell, animal experiment, Article, binding site, bioinformatics, cell differentiation, chondrocyte, chondrogenesis, chromatin immunoprecipitation, controlled study, gene expression, gene silencing, immunofluorescence test, mouse, newborn, nonhuman, promoter region, real time polymerase chain reaction, RNA interference, signal transduction, upregulation, adipose tissue, animal, antagonists and inhibitors, cell culture, chondrocyte, cytology, deficiency, drug effect, genetics, knockout mouse, metabolism, upregulation
Recommended Citation
Li, C., Zheng, Z., Zhang, X., Asatrian, G., Chen, E., Song, R., Culiat, C., Ting, K., & Soo, C. (2018). Nfatc1 Is a Functional Transcriptional Factor Mediating Nell-1-Induced Runx3 Upregulation in Chondrocytes. International Journal of Molecular Sciences, 19 (1), Article number 168-. http://dx.doi.org/10.3390/ijms19010168
Date Posted: 10 February 2023
Comments
At the time of publication, author Chenshuang Li was affiliated with the School of Dentistry, University of California. Currently, (s)he is a faculty member at the School of Dental Medicine at the University of Pennsylvania.