Departmental Papers (Dental)

Document Type

Journal Article

Date of this Version

12-15-2009

Publication Source

Journal of Immunology

Volume

183

Issue

12

Start Page

7787

Last Page

7798

DOI

10.4049/jimmunol.0902318

Abstract

Aside from the well-established self-renewal and multipotent differentiation properties, mesenchymal stem cells exhibit both immunomodulatory and anti-inflammatory roles in several experimental autoimmune and inflammatory diseases. In this study, we isolated a new population of stem cells from human gingiva, a tissue source easily accessible from the oral cavity, namely, gingiva-derived mesenchymal stem cells (GMSCs), which exhibited clonogenicity, self-renewal, and multipotent differentiation capacities. Most importantly, GMSCs were capable of immunomodulatory functions, specifically suppressed peripheral blood lymphocyte proliferation, induced expression of a wide panel of immunosuppressive factors including IL-10, IDO, inducible NO synthase (iNOS), and cyclooxygenase 2 (COX-2) in response to the inflammatory cytokine, IFN-γ. Cell-based therapy using systemic infusion of GMSCs in experimental colitis significantly ameliorated both clinical and histopathological severity of the colonic inflammation, restored the injured gastrointestinal mucosal tissues, reversed diarrhea and weight loss, and suppressed the overall disease activity in mice. The therapeutic effect of GMSCs was mediated, in part, by the suppression of inflammatory infiltrates and inflammatory cytokines/mediators and the increased infiltration of regulatory T cells and the expression of anti-inflammatory cytokine IL-10 at the colonic sites. Taken together, GMSCs can function as an immunomodulatory and anti-inflammatory component of the immune system in vivo and is a promising cell source for cell-based treatment in experimental inflammatory diseases. Copyright © 2009 by The American Association of Immunologists, Inc.

Comments

At the time of publication, author Qunzhou Zheng was affiliated with the University of Southern California, School of Dentistry. Currently, (s)he is a faculty member at the School of Dental Medicine at the University of Pennsylvania.

At the time of publication, author Shihong Shi was affiliated with the University of Southern California, School of Dentistry. Currently, (s)he is a faculty member at the School of Dental Medicine at the University of Pennsylvania.

At the time of publication, author Songtao Shi was affiliated with the University of Southern California, School of Dentistry. Currently, (s)he is a faculty member at the School of Dental Medicine at the University of Pennsylvania.

At the time of publication, author Anh D. Le was affiliated with the University of Medicine and Dentistry of New Jersey. Currently, (s)he is a faculty member at the School of Dental Medicine at the University of Pennsylvania.

Keywords

MeSH: Adult, Animals, Cells, Cultured, Coculture Techniques, Colitis, Female, Gingiva, Humans, Immunomodulation, Inflammation Mediators, Male, Mesenchymal Stem Cells, Mice, Mice, Inbred C57BL, Mice, Nude, Random Allocation EMTREE drug terms: cyclooxygenase 2, gamma interferon, indoleamine 2, 3 dioxygenase, inducible nitric oxide synthase, interleukin 10, autacoid EMTREE medical terms: animal experiment, animal model, article, cell differentiation, cell population, cell renewal, cell therapy, clonogenesis, colitis, controlled study, diarrhea, disease activity, disease severity, female, gastrointestinal mucosa, gingiva, histopathology, human, human cell, human tissue, immunomodulation, inflammation, inflammatory disease, inflammatory infiltrate, lymphocyte proliferation, male, mesenchymal stem cell, mouse, multipotent stem cell, nonhuman, priority journal, protein expression, regulatory T lymphocyte, upregulation, weight reduction, adult, animal, C57BL mouse, cell culture, coculture, colitis, cytology, immunology, immunomodulation, metabolism, nude mouse, physiology, randomization

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Date Posted: 10 February 2023

This document has been peer reviewed.