Departmental Papers (Dental)

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Journal Article

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Laboratory Investigation





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Green tea polyphenols exhibit multiple antitumor activities in various in vitro and in vivo tumor models, and the mechanisms of action are not clear. Previously, we found that green tea extract (GTE) regulates actin remodeling in different cell culture systems. Actin remodeling plays an important role in cancer cell morphology, cell adhesion, motility, and invasion. Using proteomic approaches, we found GTE-induced expression of annexin-I, a multifunctional actin binding protein, in these cell lines. In this study, we aimed to further define the functional role of GTE-induced annexin-I expression in actin remodeling, cell adhesion, and motility in lung adenocarcinoma A549 cells. We found that GTE stimulates the expression of annexin-I in a dose-dependent fashion. The GTE-induced annexin-I expression appears to be at the transcription level, and the increased annexin-I expression mediates actin polymerization, resulting in enhanced cell adhesion and decreased motility. Annexin-I specific interference resulted in loss of GTE-induced actin polymerization and cell adhesion, but not motility. In fact, annexin-I specific interference itself inhibited motility even without GTE. Together, annexin-I plays an important role in GTE-induced actin remodeling, and it may serve as a potential molecular target associated with the anticancer activities of green tea. © 2007 USCAP, Inc All rights reserved.


At the time of publication, author Anh D. Le was affiliated with the Center for Craniofacial Molecular Biology, University of Southern California School of Dentistry. Currently, (s)he is a faculty member at the School of Dental Medicine at the University of Pennsylvania.


Author keywords: Annexin-I, Cell migration, Chemoprevention, F-actin, Green tea, Lung adenocacinoma MeSH: Actins, Adenocarcinoma, Annexin A1, Anticarcinogenic Agents, Camellia sinensis, Cell Adhesion, Cell Line, Tumor, Cell Movement, Dose-Response Relationship, Drug, Gene Expression, Humans, Lung Neoplasms, Plant Extracts, Polymers, Proteomics, RNA, Messenger EMTREE drug terms: actin, green tea extract, lipocortin 1 EMTREE medical terms: actin polymerization, article, cell adhesion, cell motility, controlled study, dose response, human, human cell, lung adenocarcinoma, priority journal, protein expression, transcription regulation



Date Posted: 10 February 2023

This document has been peer reviewed.