Departmental Papers (Dental)

Document Type

Journal Article

Date of this Version

9-2013

Publication Source

Journal of Dental Research

Volume

92

Issue

9

Start Page

825

Last Page

832

DOI

10.1177/0022034513497961

Abstract

Gingivae represent a unique soft tissue that serves as a biological barrier to cover the oral cavity side of the maxilla and mandible. Recently, the gingivae were identified as containing mesenchymal stem cells (GMSCs). However, it is unknown whether the GMSCs are derived from cranial neural crest cells (CNCC) or the mesoderm. In this study, we show that around 90% of GMSCs are derived from CNCC and 10% from the mesoderm. In comparison with mesoderm MSCs (M-GMSCs), CNCC-derived GMSCs (N-GMSCs) show an elevated capacity to differentiate into neural cells and chondrocytes and induce activated T-cell apoptosis in vitro. When transplanted into mice with dextran sulfate sodium (DSS)-induced colitis, N-GMSCs showed superior effects in ameliorating inflammatory-related disease phenotype in comparison with the M-GMSC treatment group. Mechanistically, the increased immunomodulatory effect of N-GMSCs is associated with up-regulated expression of FAS ligand (FASL), a transmembrane protein that plays an important role in MSC-based immunomodulation. In summary, our study indicates that the gingivae contain both neural-crest- and mesoderm-derived MSCs with distinctive stem cell properties. © International & American Associations for Dental Research.

Keywords

apoptosis, differentiation, gingiva, immunomodulation, mesenchymal stem cells, neural crest cells, Animals, Apoptosis, Body Weight, Cell Count, Cell Culture Techniques, Cell Differentiation, Cell Lineage, Chondrocytes, Coculture Techniques, Colitis, Dextran Sulfate, Fas Ligand Protein, Gingiva, Immunomodulation, Lymphocyte Activation, Mesenchymal Stem Cell Transplantation, Mesenchymal Stromal Cells, Mesoderm, Mice, Mice, Inbred C57BL, Mice, Inbred Strains, Mice, Transgenic, Neural Crest, Neurons, T-Lymphocytes, Up-Regulation, dextran sulfate, Fas ligand, Fasl protein, mouse, animal, apoptosis, article, body weight, C57BL mouse, cartilage cell, cell count, cell differentiation, cell lineage, chemically induced disorder, classification, coculture, colitis, comparative study, culture technique, cytology, differentiation, gingiva, immunology, immunomodulation, lymphocyte activation, mesenchymal stem cell, mesenchymal stem cell transplantation, mesenchymal stroma cell, mesoderm, metabolism, mouse, mouse strain, nerve cell, neural crest, neural crest cells, physiology, T lymphocyte, transgenic mouse, upregulation

Download

Share

COinS
 

Date Posted: 10 February 2023

This document has been peer reviewed.