Date of this Version
Restorative Dentistry & Endodontics
The biocompatibility of two experimental scaffolds for potential use in revascularization or pulp regeneration was evaluated.
Materials and Methods
One resilient lyophilized collagen scaffold (COLL), releasing metronidazole and clindamycin, was compared to an experimental injectable poly(lactic-co-glycolic) acid scaffold (PLGA), releasing clindamycin. Human dental pulp stem cells (hDPSCs) were seeded at densities of 1.0 × 104, 2.5 × 104, and 5.0 × 104. The cells were investigated by light microscopy (cell morphology), MTT assay (cell proliferation) and a cytokine (IL-8) ELISA test (biocompatibility).
Under microscope, the morphology of cells coincubated for 7 days with the scaffolds appeared healthy with COLL. Cells in contact with PLGA showed signs of degeneration and apoptosis. MTT assay showed that at 5.0 × 104 hDPSCs, COLL demonstrated significantly higher cell proliferation rates than cells in media only (control, p < 0.01) or cells co-incubated with PLGA (p < 0.01). In ELISA test, no significant differences were observed between cells with media only and COLL at 1, 3, and 6 days. Cells incubated with PLGA expressed significantly higher IL-8 than the control at all time points (p < 0.01) and compared to COLL after 1 and 3 days (p < 0.01).
The COLL showed superior biocompatibility and thus may be suitable for endodontic regeneration purposes.
Made available with a CC BY-NC 4.0 license.
clindamycin, matrix, metronidazole, regenerative endodontics, revascularization, scaffold
Leong, D., Setzer, F., Trope, M., & Karabucak, B. (2016). Biocompatibility of two Experimental Scaffolds for Regenerative Endodontics. Restorative Dentistry & Endodontics, 41 (2), 98-105. http://dx.doi.org/10.5395/rde.2016.41.2.98
Date Posted: 23 July 2018
This document has been peer reviewed.