Departmental Papers (Dental)

Document Type

Journal Article

Date of this Version

4-1-2015

Publication Source

Tissue Engineering - Part A.

Volume

21

Issue

7-8

Start Page

1388

Last Page

1397

DOI

10.1089/ten.tea.2014.0280

Copyright/Permission Statement

Scaffolds play an important role in directing three-dimensional (3D) cartilage regeneration. Our recent study reported the potential advantages of bone marrow clots (MC) in promoting extracellular matrix (ECM) scaffold chondrogenic regeneration. The aim of this study is to build a new scaffold for MC, with improved characteristics in mechanics, shaping, and biodegradability, compared to our previous study. To address this issue, this study prepared a 3D porous polycaprolactone (PCL)-hydroxyapatite (HA) scaffold combined with MC (Group A), while the control group (Group B) utilized a bone marrow stem cell seeded PCL-HA scaffold. The results of in vitro cultures and in vivo implantation demonstrated that although an initial obstruction of nutrient exchange caused by large amounts of fibrin and erythrocytes led to a decrease in the ratio of live cells in Group A, these scaffolds also showed significant improvements in cell adhesion, proliferation, and chondrogenic differentiation with porous recanalization in the later culture, compared to Group B. After 4 weeks of in vivo implantation, Group A scaffolds have a superior performance in DNA content, Sox9 and RunX2 expression, cartilage lacuna-like cell and ECM accumulation, when compared to Group B. Furthermore, Group A scaffold size and mechanics were stable during in vitro and in vivo experiments, unlike the scaffolds in our previous study. Our results suggest that the combination with MC proved to be a highly efficient, reliable, and simple new method that improves the biological performance of 3D PCL-HA scaffold. The MC-PCL-HA scaffold is a candidate for future cartilage regeneration studies. © Copyright 2015, Mary Ann Liebert, Inc. 2015.

Comments

At the time of publication, author Chenshuang Li was affiliated with the Dental and Craniofacial Research Institute, University of California and the School and Hospital of Stomatology, Peking University. Currently, (s)he is a faculty member at the School of Medical Dentistry at the University of Pennsylvania.

Keywords

Animals, Bone Marrow Cells, Cells, Cultured, Chondrogenesis, Durapatite, Female, Gene Expression Regulation, Implants, Experimental, Polyesters, Porosity, Printing, Three-Dimensional, Rabbits, Regeneration, Stem Cells, Tissue Scaffolds, Lacuna, 3D printers, Biodegradability, Bone, Cartilage, Cell adhesion, Hydroxyapatite, Stem cells, aggrecan, collagen type 2, fibrin, glyceraldehyde 3 phosphate dehydrogenase, glycosaminoglycan, hydroxyapatite, polycaprolactone, porous polymer, transcription factor RUNX2, transcription factor Sox9, hydroxyapatite, polycaprolactone, polyester, Biological performance, Bone marrow stem cells, Cartilage regeneration, Chondrogenic differentiation, Extracellular matrices, Nutrient exchange, Recanalization, Threedimensional (3-d), animal cell, animal experiment, animal tissue, Article, biomechanics, bone marrow, bone marrow clot, bone marrow derived mesenchymal stem cell, cartilage cell, cartilage regeneration, cell adhesion, cell proliferation, chondrogenesis, controlled study, DNA content, erythrocyte, extracellular matrix, female, mouse, New Zealand White (rabbit), nonhuman, nude mouse, priority journal, three dimensional printing, tissue differentiation, tissue regeneration, tissue scaffold, animal, bone marrow cell, cell culture, chemistry, chondrogenesis, cytology, drug effects, gene expression regulation, implant, porosity, rabbit, regeneration, stem cell, tissue scaffold, Scaffolds (biology)

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Date Posted: 10 February 2023

This document has been peer reviewed.