Departmental Papers (Dental)
Date of this Version
Journal of Dental Research
Stem cells from human exfoliated deciduous teeth have been identified as a new post-natal stem cell population with multipotential differentiation capabilities, including regeneration of mineralized tissues in vivo. To examine the efficacy of utilizing these stem cells in regenerating orofacial bone defects, we isolated stem cells from miniature pig deciduous teeth and engrafted the critical-size bone defects generated in swine mandible models. Our results indicated that stem cells from miniature pig deciduous teeth, an autologous and easily accessible stem cell source, were able to engraft and regenerate bone to repair critical-size mandibular defects at 6 months post-surgical reconstruction. This pre-clinical study in a large-animal model, specifically swine, allows for testing of a stem cells/scaffold construct in the restoration of orofacial skeletal defects and provides rapid translation of stem-cell-based therapy in orofacial reconstruction in human clinical trials.
Author keywords: Bone, Deciduous tooth, Miniature pig, Stem cell, Tissue engineering MeSH: Animals, Bone Regeneration, Cells, Cultured, Dental Pulp, Disease Models, Animal, Feasibility Studies, Female, Green Fluorescent Proteins, Guided Tissue Regeneration, Mandibular Diseases, Microscopy, Fluorescence, Osteogenesis, Reconstructive Surgical Procedures, Stem Cell Transplantation, Stem Cells, Swine, Swine, Miniature, Tissue Engineering, Tissue Scaffolds, Tomography, X-Ray Computed, Tooth, Deciduous, Transplantation, Autologous EMTREE drug terms: diagnostic agent, green fluorescent protein, tissue scaffold EMTREE medical terms: animal, article, autotransplantation, bone development, bone regeneration, cell culture, computer assisted tomography, cytology, deciduous tooth, disease model, feasibility study, female, fluorescence microscopy, jaw disease, methodology, minipig, physiology, plastic surgery, stem cell, stem cell transplantation, swine, tissue engineering, tissue regeneration, tooth pulp
Zheng, Y., Liu, Y., Zhang, C. M., Li, W. H., Shi, S., Le, A. D., & Wang, S. L. (2009). Stem Cells from Deciduous Tooth Repair Mandibular Defect in Swine. Journal of Dental Research, 88 (3), 249-254. http://dx.doi.org/10.1177/0022034509333804
Endodontics and Endodontology Commons, Oral and Maxillofacial Surgery Commons, Oral Biology and Oral Pathology Commons, Periodontics and Periodontology Commons
Date Posted: 09 February 2023
This document has been peer reviewed.
At the time of publication, author Shihong Shi was affiliated with the University of Southern California, School of Dentistry. Currently, (s)he is a faculty member at the School of Dental Medicine at the University of Pennsylvania.
At the time of publication, author Anh D. Le was affiliated with the University of Southern California, School of Dentistry. Currently, (s)he is a faculty member at the School of Dental Medicine at the University of Pennsylvania.