Date of this Version
Journal of Biological Chemistry
Background: A repeats-in-toxin (RTX) leukotoxin and its integrin receptor aggregate in cholesterol-rich lipid rafts. Results: The affinity of the toxin to cholesterol is driven by a cholesterol recognition/amino acid consensus (CRAC) motif. Conclusion: Leukotoxin cytotoxicity is regulated by the CRAC motif. Significance: Other RTX toxins contain this CRAC motif, suggesting a role for cholesterol recognition in RTX cytolysis. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.
Amino Acid Motifs; Bacterial Toxins; Cholesterol; Exotoxins; Humans; Jurkat Cells; Lymphocyte Function-Associated Antigen-1; Membrane Microdomains; Pasteurellaceae; Protein Binding; Surface Plasmon Resonance
Brown, A. C., Balashova, A. C., Epand, R. M., Epand, R. F., Bragin, A., Kachlany, S. C., Walters, M. J., Du, Y., Boesze-Battaglia, K., & Lally, E. T. (2013). Aggregatibacter Actinomycetemcomitans Leukotoxin Utilizes a Cholesterol Recognition/Amino Acid Consensus Site for Membrane Association. Journal of Biological Chemistry, 288 (32), 23607-23621. http://dx.doi.org/10.1074/jbc.M113.486654
Date Posted: 08 December 2022
This document has been peer reviewed.