Departmental Papers (Dental)
Document Type
Journal Article
Date of this Version
4-17-2009
Publication Source
Journal of Biological Chemistry
Volume
284
Issue
16
Start Page
10877
Last Page
10889
DOI
10.1074/jbc.M808857200
Abstract
Melanoregulin (MREG), the product of the Mregdsu gene, is a small highly charged protein, hypothesized to play a role in organelle biogenesis due to its effect on pigmentation in dilute, ashen, and leaden mutant mice. Here we provide evidence that MREG is required in lysosome-dependent phagosome degradation. In the Mreg-/- mouse, we show that loss of MREG function results in phagosome accumulation due to delayed degradation of engulfed material. Over time, the Mreg-/- mouse retinal pigment epithelial cells accumulate the lipofuscin component, A2E. MREG-deficient human and mouse retinal pigment epithelial cells exhibit diminished activity of the lysosomal hydrolase, cathepsin D, due to defective processing. Moreover, MREG localizes to small intracellular vesicles and associates with the endosomal phosphoinositide, phosphatidylinositol 3,5-biphosphate. Collectively, these studies suggest that MREG is required for lysosome maturation and support a role for MREG in intracellular trafficking.
Recommended Citation
Damek-Poprawa, M., Diemer, T., Lopes, V. S., Lillo, C., Harper, D. C., Marks, M. S., Yalin, W., Sparrow, J. R., Rachel, R. A., Williams, D. S., & Boesze-Battaglia, K. (2009). Melanoregulin (MREG) Modulates Lysosome Function in Pigment Epithelial Cells. Journal of Biological Chemistry, 284 (16), 10877-10889. http://dx.doi.org/10.1074/jbc.M808857200
Date Posted: 08 December 2022
This document has been peer reviewed.