Departmental Papers (Dental)

Document Type

Journal Article

Date of this Version

12-2021

Publication Source

Communications Biology

Volume

4

Issue

1

Start Page

Article number 879

DOI

10.1038/s42003-021-02392-8

Abstract

The rostral migratory stream (RMS) facilitates neuroblast migration from the subventricular zone to the olfactory bulb throughout adulthood. Brain lesions attract neuroblast migration out of the RMS, but resultant regeneration is insufficient. Increasing neuroblast migration into lesions has improved recovery in rodent studies. We previously developed techniques for fabricating an astrocyte-based Tissue-Engineered RMS (TE-RMS) intended to redirect endogenous neuroblasts into distal brain lesions for sustained neuronal replacement. Here, we demonstrate that astrocyte-like-cells can be derived from adult human gingiva mesenchymal stem cells and used for TE-RMS fabrication. We report that key proteins enriched in the RMS are enriched in TE-RMSs. Furthermore, the human TE-RMS facilitates directed migration of immature neurons in vitro. Finally, human TE-RMSs implanted in athymic rat brains redirect migration of neuroblasts out of the endogenous RMS. By emulating the brain’s most efficient means for directing neuroblast migration, the TE-RMS offers a promising new approach to neuroregenerative medicine. © 2021, This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.

Keywords

Animals, Astrocytes, Humans; Male, Neural Stem Cells, Neurogenesis, Neurons, Rats, Rats, Nude, Tissue Engineering, animal; astrocyte; human; male; nerve cell; nervous system development; neural stem cell, nude rat, physiology, rat, tissue engineering, transplantation

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Date Posted: 08 June 2022

This document has been peer reviewed.