Departmental Papers (Dental)

Document Type

Journal Article

Date of this Version

1-1985

Publication Source

Journal of Investigative Dermatology

Volume

84

Issue

1

Start Page

22

Last Page

26

DOI

10.1111/1523-1747.ep12274607

Abstract

A method has been developed for the enzymic dissociation of rat skin into its component cells. The resulting suspensions contained 3–5% mast cells. The latter were intact as judged by light microscopy and exhibited a low spontaneous release of histamine. Cells obtained from actively sensitized animals released histamine on challenge with specific antigen. The process was rapid, being essentially complete within 1 mm and was both calcium-and temperature-dependent. The cells also responded to antirat IgE and to calcium ionophores but showed a selective, time-dependent reactivity toward defined chemical histamine liberators. On the basis of these results the properties of the cutaneous mast cell are compared with those previously reported for mastocytes from other sources and discussed in terms of the general heterogeneity of this cell population.

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Date Posted: 01 March 2022

This document has been peer reviewed.