Departmental Papers (Dental)

Title

Mouse Mandible Contains Distinctive Mesenchymal Stem Cells

Document Type

Journal Article

Date of this Version

3-2011

Publication Source

Journal of Dental Research

Volume

90

Issue

3

Start Page

317

Last Page

324

DOI

10.1177/0022034510387796

Abstract

Although human orofacial bone-marrow-derived mesenchymal stem cells showed differentiation traits distinctly different from those of mesenchymal stem cells (MSCs) derived from long bone marrow (BMMSCs), mouse MSCs derived from orofacial bone have not been isolated due to technical difficulties, which in turn precludes the use of mouse models to study and cure orofacial diseases. In this study, we developed techniques to isolate and expand mouse orofacial bone/bone-marrow-derived MSCs (OMSCs) from mandibles and verified their MSC characteristics by single-colony formation, multi-lineage differentiation, and in vivo tissue regeneration. Activated T-lymphocytes impaired OMSCs via the Fas/Fas ligand pathway, as occurs in BMMSCs. Furthermore, we found that OMSCs are distinct from BMMSCs with respect to regulating T-lymphocyte survival and proliferation. Analysis of our data suggests that OMSCs are a unique population of MSCs and play an important role in systemic immunity. Abbreviations: BMMSC, bone marrow mesenchymal stem cell; HA/TCP, hydroxyapatite/tricalcium phosphate; OMSC, orofacial mesenchymal stem cell; OVX, ovariectomized.

Copyright/Permission Statement

Yamaza, T., Ren, G., Akiyama, K., Chen, C., Shi, Y., & Shi, S. (2011). Mouse mandible contains distinctive mesenchymal stem cells. Journal of Dental Research, 90(3), 317-324. doi:10.1177/0022034510387796. Copyright © [2011] (Journal of Dental Research). Reprinted by permission of SAGE Publications

Comments

At the time of publication, author Songtao Shi was affiliated with the University of Southern California. Currently, he is a faculty member at the Dental School at the University of Pennsylvania.

Keywords

mesenchymal stem cells, mouse mandible, differentiation, tissue regeneration

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Date Posted: 01 March 2022

This document has been peer reviewed.