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Current Opinion in Chemical Biology
After approval of the first plant-made biopharmaceutical by FDA for human use, many protein drugs are now in clinical development. Within the last decade, significant advances have been made in expression of heterologous complex/large proteins in chloroplasts of edible plants using codon optimized human or viral genes. Furthermore, advances in quantification enable determination of in-planta drug dosage. Oral delivery of plastid-made biopharmaceuticals (PMB) is affordable because it eliminates prohibitively expensive fermentation, purification processes addressing major challenges of short shelf-life after cold storage. In this review, we discuss recent advances in PMBs against metabolic, inherited or infectious diseases, and also mechanisms of post-translational modifications (PTM) in order to increase our understanding of functional PMBs.
© <2017>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Zhang, B., Shanmugaraj, B., & Daniell, H. (2017). Expression and Functional Evaluation of Biopharmaceuticals Made in Plant Chloroplasts. Current Opinion in Chemical Biology, 38 17-23. http://dx.doi.org/10.1016/j.cbpa.2017.02.007
Date Posted: 01 March 2022