Date of this Version
FEMS Microbiology Letters
Chimeras composed of the cdtB gene of a novel bacterial genotoxin and the human type I DNAse I gene were constructed and their products characterized relative to the biochemical and enzymatic properties of the native proteins. The product of a cdtB/DNAse I chimera formed a heterotrimer with the CdtA and CdtC subunits of the genotoxin, and targeted mutations increased the specific activity of the hybrid protein. Expression of active chimeric gene products established that the CdtB protein is an atypical divalent cation-dependent endonuclease and demonstrated the potential for genetically engineering a new class of therapeutic agent for inhibiting the proliferation of cancer cells.
This is a pre-copyedited, author-produced PDF of an article accepted for publication in FEMS Microbiology Letters following peer review. The version of this record is available online at 10.1111/j.1574-6968.2008.01457.x.
Aggregatibacter actinomycetemcomitans, chimera, cytolethal distending toxin, epithelial cells, pathogen, type I DNAse
DiRienzo, J. M., Cao, L., Volgina, A., Bandelac, G., & Korostoff, J. M. (2009). Functional and Structural Characterization of Chimeras of a Bacterial Genotoxin and Human Type I DNAse. FEMS Microbiology Letters, 291 (2), 222-231. http://dx.doi.org/10.1111/j.1574-6968.2008.01457.x
Date Posted: 06 March 2015
This document has been peer reviewed.