Departmental Papers (Dental)

Document Type

Journal Article

Date of this Version

3-2007

Publication Source

Plant Biotechnology Journal

Volume

5

Issue

2

Start Page

230

Last Page

239

DOI

10.1111/j.1467-7652.2006.00234.x

Abstract

Chloroplast genetic engineering offers several advantages, including high levels of transgene expression, transgene containment via maternal inheritance and multigene engineering in a single transformation event. Entamoeba histolytica infects 50 million people, causing about 100 000 deaths annually, but there is no approved vaccine against this pathogen. LecA, a potential target for blocking amoebiasis, was expressed for the first time in transgenic plants. Stable transgene integration into chloroplast genomes and homoplasmy were confirmed by polymerase chain reaction and Southern blot analyses. LecA expression was evaluated by Western blots and quantified by enzyme-linked immunosorbent assay (up to 6.3% of total soluble protein or 2.3 mg LecA/g leaf tissue). Subcutaneous immunization of mice with crude extract of transgenic leaves resulted in higher immunoglobulin G titres (up to 1 : 10 000) than in previous reports. An average yield of 24 mg of LecA per plant should produce 29 million doses of vaccine antigen per acre of transgenic plants. Such high levels of expression and immunogenicity should facilitate the development of a less expensive amoebiasis vaccine.

Copyright/Permission Statement

This is the peer reviewed version of the following article: Chebolu, S., & Daniell, H. (2007). Stable expression of Gal/GalNAc lectin of Entamoeba histolytica in transgenic chloroplasts and immunogenicity in mice towards vaccine development for amoebiasis. Plant Biotechnology Journal, 5(2), 230–239. http://doi.org/10.1111/j.1467-7652.2006.00234.x, which has been published in final form at http://doi.org/10.1111/j.1467-7652.2006.00234.x. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving http://olabout.wiley.com/WileyCDA/Section/id-820227.html#terms.

Comments

At the time of publication, author Henry Daniell was affiliated with the University of Central Florida. Currently, he is a faculty member at the School of Dental Medicine at the University of Pennsylvania.

Keywords

amoebiasis vaccine, genetically modified crops, plant-made vaccines, tobacco

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Dentistry Commons

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Date Posted: 01 March 2022

This document has been peer reviewed.