Departmental Papers (Dental)

Document Type

Journal Article

Date of this Version

4-17-2007

Publication Source

Proc Natl Acad Sci U S A

Volume

104

Issue

17

Start Page

6879

Last Page

6880

DOI

10.1073/pnas.0702219104

Abstract

Transgene containment is a central concern in genetically modified (GM) crops, especially for those with out-crossing wild relatives. However, plant cells contain genomes in the nucleus, mitochondria, and chloroplasts. Whereas nuclear genes are biparentally inherited, organelle genes are in general maternally inherited (1, 2). Therefore, engineering foreign genes in the chloroplast genome may provide containment from pollen transmission. This topic was hotly debated when the first herbicide-resistant (Round-Up-ready) transplastomic plants (transformed with chloroplast genomes) were reported (3). In this issue of PNAS, two articles highlight different aspects of this important question. The article by Svab and Maliga (4) examines whether alien cytoplasm contributes to rare paternal plastid transmission. They report low frequencies (10−4 to 10−5) of paternal plastids in both normal and alien cytoplasm and caution that this observation is biased by tissue culture selection, and therefore transgenes are less likely to get into the germ line under field conditions. Most importantly, these investigators show that the entire plastid genome is transmitted by pollen instead of plastid DNA fragments and that the mitochondria are also cotransmitted. In a parallel study, Ruf et al. (5) set up a stringent selection system for paternal transmission by using male-sterile maternal parents and transplastomic pollen donors, conferring plastid-specific antibiotic resistance and green fluorescence for visual screening. This selection system identified 6 among 2.1 million seedlings screened (frequency of 2.86 × 10−6) that showed paternal transmission of transgenes, and the authors concluded that plastid transformation provides an effective tool to increase biosafety of GM crops.

Copyright/Permission Statement

© [2007] National Academy of Sciences.

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Date Posted: 01 March 2022

This document has been peer reviewed.