Date of this Version
Journal of Pediatric Surgery
We aimed at determining whether osseous grafts engineered from amniotic mesenchymal stem cells (aMSCs) could be employed in postnatal sternal repair.
Leporine aMSCs were isolated, identified, transfected with green fluorescent protein (GFP), expanded, and seeded onto biodegradable electrospun nanofibrous scaffolds (n=6). Constructs were dynamically maintained in an osteogenic medium and equally divided into two groups with respect to time in vitro, namely 14.6 or 33.9 weeks. They were then used to repair full thickness sternal defects spanning 2–3 intercostal spaces in allogeneic kits (n=6). Grafts were submitted to multiple analyses 2 months thereafter.
Chest roentgenograms showed defect closure in all animals, confirmed at necropsy. Graft density as assessed by micro-CT scans increased significantly in vivo, yet there were no differences in mineralization by extracellular calcium measurements pre- and post-implantation. There was a borderline increase in alkaline phosphatase activity in vivo, suggesting ongoing graft remodeling. Histologically, implants contained GFP-positive cells and few mononuclear infiltrates. There were no differences between the two construct groups in any comparison.
Engineered osseous grafts derived from amniotic mesenchymal stem cells may become a viable alternative for sternal repair. The amniotic fluid can be a practical cell source for engineered chest wall reconstruction.
© <2009>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
tissue engineering, amniotic mesenchymal stem cells, mesenchymal stem cells, amniotic fluid, chest wall anomalies, nanofibers, fetus, sternum, congenital anomalies
Steigman, S. A., Ahmed, A., Shanti, R. M., Tuan, R. S., Valim, C., & Fauza, D. O. (2009). Sternal Repair with Bone Grafts Engineered from Amniotic Mesenchymal Stem Cells. Journal of Pediatric Surgery, 44 (6), 1120-1126. http://dx.doi.org/10.1016/j.jpedsurg.2009.02.038
Date Posted: 01 March 2022
This document has been peer reviewed.