Departmental Papers (Dental)

Document Type

Journal Article

Date of this Version

6-2009

Publication Source

Journal of Pediatric Surgery

Volume

44

Issue

6

Start Page

1120

Last Page

1126

DOI

10.1016/j.jpedsurg.2009.02.038

Abstract

Background

We aimed at determining whether osseous grafts engineered from amniotic mesenchymal stem cells (aMSCs) could be employed in postnatal sternal repair.

Methods

Leporine aMSCs were isolated, identified, transfected with green fluorescent protein (GFP), expanded, and seeded onto biodegradable electrospun nanofibrous scaffolds (n=6). Constructs were dynamically maintained in an osteogenic medium and equally divided into two groups with respect to time in vitro, namely 14.6 or 33.9 weeks. They were then used to repair full thickness sternal defects spanning 2–3 intercostal spaces in allogeneic kits (n=6). Grafts were submitted to multiple analyses 2 months thereafter.

Results

Chest roentgenograms showed defect closure in all animals, confirmed at necropsy. Graft density as assessed by micro-CT scans increased significantly in vivo, yet there were no differences in mineralization by extracellular calcium measurements pre- and post-implantation. There was a borderline increase in alkaline phosphatase activity in vivo, suggesting ongoing graft remodeling. Histologically, implants contained GFP-positive cells and few mononuclear infiltrates. There were no differences between the two construct groups in any comparison.

Conclusions

Engineered osseous grafts derived from amniotic mesenchymal stem cells may become a viable alternative for sternal repair. The amniotic fluid can be a practical cell source for engineered chest wall reconstruction.

Copyright/Permission Statement

© <2009>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/

Comments

At the time of publication, author Rabie Shanti was affiliated with Children's Hospital Boston and Harvard Medical School. Currently, he is a faculty member at the School of Dental Medicine at the University of Pennsylvania

Keywords

tissue engineering, amniotic mesenchymal stem cells, mesenchymal stem cells, amniotic fluid, chest wall anomalies, nanofibers, fetus, sternum, congenital anomalies

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Date Posted: 01 March 2022

This document has been peer reviewed.