Departmental Papers (Dental)

Document Type

Journal Article

Date of this Version

11-2005

Publication Source

Journal of Dental Research

Volume

84

Issue

11

Start Page

994

Last Page

998

DOI

10.1177/154405910508401105

Abstract

Post-natal human dental pulp stem cells (DPSCs) represent a unique precursor population in the dental pulp, which has multipotential and can regenerate a dentin/pulp-like structure. Because the dental pulp is frequently infected by oral bacteria due to dental decay, in this study, we examined whether lipopolysaccharide (LPS) and tumor necrosis factor (TNF) activated the immunologic transcription factor nuclear factor kappa B (NF-κB) in DPSCs. We found that both TNF and LPS activated the I-kappa B kinase complex (IKK) in DPSCs to induce the phosphorylation and degradation of IκBα, resulting in the nuclear translocation of NF-κB. Consistently, both TNF and LPS rapidly induced the expression of the NF-κB-dependent gene interleukin-8 (IL-8). However, unlike in monocytes, we found that LPS could not induce the phosphorylation of the NF-κB active subunit p65 in DPSCs. In summary, our studies suggest that DPSCs may be involved in immune responses during pulpal infection through activating NF-κB.

Copyright/Permission Statement

Chang, J., Zhang, C., Tani-Ishii, N., Shi, S., & Wang, C. (2005). NF-kappaB activation in human dental pulp stem cells by TNF and LPS. Journal of Dental Research, 84(11), 994.Copyright © [2005] (Journal of Dental Research). Reprinted by permission of SAGE Publications.

Comments

At the time of publication, author Songtao Shi was affiliated with the National Institute of Dental and Craniofacial Research. Currently, he is a faculty member at the School of Dental Medicine at the University of Pennsylvania

Keywords

NF-κB, dental pulp stem cells, tumor necrosis factor, LPS, inflammation

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Date Posted: 01 March 2022

This document has been peer reviewed.