Departmental Papers (Dental)

Document Type

Journal Article

Date of this Version

2-2009

Publication Source

FEMS Microbiology Letters

Volume

291

Issue

2

Start Page

222

Last Page

231

DOI

10.1111/j.1574-6968.2008.01457.x

Abstract

Chimeras composed of the cdtB gene of a novel bacterial genotoxin and the human type I deoxyribonuclease (DNase I) gene were constructed and their products characterized relative to the biochemical and enzymatic properties of the native proteins. The product of a cdtB/DNase I chimera formed a heterotrimer with the CdtA and CdtC subunits of the genotoxin and targeted mutations increased the specific activity of the hybrid protein. Expression of active chimeric gene products established that the CdtB protein is an atypical divalent cation-dependent endonuclease and demonstrated the potential for genetically engineering a new class of therapeutic agent for inhibiting the proliferation of cancer cells.

Copyright/Permission Statement

This article has been accepted for publication in FEMS Microbiology Letters Published by Oxford University Press.

Keywords

Aggregatibacter actinomycetemcomitans, chimera, cytolethal distending toxin, epithelial cells, genotoxin, pathogen, type I deoxyribonuclease

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Date Posted: 01 March 2022

This document has been peer reviewed.