Departmental Papers (Dental)
Document Type
Journal Article
Date of this Version
2-2009
Publication Source
FEMS Microbiology Letters
Volume
291
Issue
2
Start Page
222
Last Page
231
DOI
10.1111/j.1574-6968.2008.01457.x
Abstract
Chimeras composed of the cdtB gene of a novel bacterial genotoxin and the human type I deoxyribonuclease (DNase I) gene were constructed and their products characterized relative to the biochemical and enzymatic properties of the native proteins. The product of a cdtB/DNase I chimera formed a heterotrimer with the CdtA and CdtC subunits of the genotoxin and targeted mutations increased the specific activity of the hybrid protein. Expression of active chimeric gene products established that the CdtB protein is an atypical divalent cation-dependent endonuclease and demonstrated the potential for genetically engineering a new class of therapeutic agent for inhibiting the proliferation of cancer cells.
Keywords
Aggregatibacter actinomycetemcomitans, chimera, cytolethal distending toxin, epithelial cells, genotoxin, pathogen, type I deoxyribonuclease
Recommended Citation
DiRienzo, J. M., Cao, L., Volgina, A., Bandelac, G., & Korostoff, J. (2009). Functional and Structural Characterization of Chimeras of a Bacterial Genotoxin and Human Type I Deoxyribonuclease. FEMS Microbiology Letters, 291 (2), 222-231. http://dx.doi.org/10.1111/j.1574-6968.2008.01457.x
Date Posted: 01 March 2022
This document has been peer reviewed.