Departmental Papers (Dental)
Basic Fibroblast Growth Factor Inhibits Osteogenic Differentiation of SHED through ERK Signaling
Date of this Version
Stem cells from human exfoliated deciduous teeth (SHED) are a unique postnatal stem cell population capable of regenerating mineralized tissue and treating immune disorders. However, the mechanism that controls SHED differentiation is not fully understood. Here, we showed that basic fibroblast growth factor (bFGF) treatment attenuated SHED-mediated mineralized tissue regeneration through activation of the extracellular signal-regulated kinase (ERK) 1/2 pathway.
Material and Method
The level of mineralized nodule formation was assessed by alizarin red staining. Expression levels of osteogenic genes, OCN and Runx2, were examined by RT-PCR. Subcutaneous implantation approach was used to assess in vivo bone formation. Downstream signaling pathways of bFGF were examined by Western blotting.
Activation of ERK1/2 signaling by bFGF treatment inhibited WNT/β-catenin pathway, leading to osteogenic deficiency of SHED. ERK1/2 inhibitor treatment rescued bFGF-induced osteogenic differentiation deficiency.
These data suggest that bFGF inhibits osteogenic differentiation of SHED via ERK1/2 pathway. Blockade ERK1/2 signaling by small molecular inhibitor-treatment improves bone formation of SHED after bFGF treatment.
This is the pre-peer reviewed version of the following article: [Li, B., Qu, C., Chen, C., Liu, Y., Akiyama, K., Yang, R., . . . Shi, S. (2012). Basic fibroblast growth factor inhibits osteogenic differentiation of stem cells from human exfoliated deciduous teeth through ERK signaling. Oral Diseases, 18(3), 285-292. doi:10.1111/j.1601-0825.2011.01878.x], which has been published in final form at [http://dx.doi.org/10.1111/j.1601-0825.2011.01878.x]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
SHED, ERK, differentiation, tissue regeneration
Li, B., Qu, C., Chen, C., Liu, Y., Akiyama, K., Yang, R., Chen, F., Zhao, Y., & Shi, S. (2012). Basic Fibroblast Growth Factor Inhibits Osteogenic Differentiation of SHED through ERK Signaling. Oral Diseases, 18 (3), 285-292. http://dx.doi.org/10.1111/j.1601-0825.2011.01878.x
Date Posted: 01 March 2022
This document has been peer reviewed.
At the time of publication, author Chider Chen was affiliated with the University of Southern California. Currently, he is a faculty member at the School of the Dental Medicine at the University of Pennsylvania.